AA 5288 then AA 6052 at Charlotte
Washington National → Charlotte → Tallahassee
Timetable, Charlotte time
14:37AA 5288 from Washington National at the gate
14:20AA 6052 to Tallahassee leaves the gate
23 h 43 apart on the timetable, overnight
AA 6052 leaves 23 h 43 after AA 5288 arrives on the timetable: more than half a day, so not a connection we count.