AA 5307 then AA 2255 at Charlotte
Louisville → Charlotte → Pittsburgh
Timetable, Charlotte time
10:25AA 5307 from Louisville at the gate
08:55AA 2255 to Pittsburgh leaves the gate
22 h 30 apart on the timetable, overnight
AA 2255 leaves 22 h 30 after AA 5307 arrives on the timetable: more than half a day, so not a connection we count.