AA 5332 then AA 2255 at Charlotte
Washington → Charlotte → Pittsburgh
Timetable, Charlotte time
11:08AA 5332 from Washington at the gate
08:55AA 2255 to Pittsburgh leaves the gate
21 h 47 apart on the timetable, overnight
AA 2255 leaves 21 h 47 after AA 5332 arrives on the timetable: more than half a day, so not a connection we count.