AA 5332 then AA 2276 at Charlotte
Washington → Charlotte → Orlando
Timetable, Charlotte time
11:08AA 5332 from Washington at the gate
10:47AA 2276 to Orlando leaves the gate
23 h 39 apart on the timetable, overnight
AA 2276 leaves 23 h 39 after AA 5332 arrives on the timetable: more than half a day, so not a connection we count.