AA 5332 then AA 6125 at Charlotte
Washington → Charlotte → Columbia Metropolitan
Timetable, Charlotte time
12:22AA 5332 from Washington at the gate
12:15AA 6125 to Columbia Metropolitan leaves the gate
23 h 53 apart on the timetable, overnight
AA 6125 leaves 23 h 53 after AA 5332 arrives on the timetable: more than half a day, so not a connection we count.