AA 5353 then AA 1643 at Charlotte
Wilmington → Charlotte → Charleston (South Carolina)
Timetable, Charlotte time
15:16AA 5353 from Wilmington at the gate
12:33AA 1643 to Charleston (South Carolina) leaves the gate
21 h 17 apart on the timetable, overnight
AA 1643 leaves 21 h 17 after AA 5353 arrives on the timetable: more than half a day, so not a connection we count.