AA 5353 then AA 2044 at Charlotte
Wilmington → Charlotte → Hamilton
Timetable, Charlotte time
15:16AA 5353 from Wilmington at the gate
11:30AA 2044 to Hamilton leaves the gate
20 h 14 apart on the timetable, overnight
AA 2044 leaves 20 h 14 after AA 5353 arrives on the timetable: more than half a day, so not a connection we count.