AA 5353 then AA 3091 at Charlotte
Wilmington → Charlotte → Nashville
Timetable, Charlotte time
15:16AA 5353 from Wilmington at the gate
11:55AA 3091 to Nashville leaves the gate
20 h 39 apart on the timetable, overnight
AA 3091 leaves 20 h 39 after AA 5353 arrives on the timetable: more than half a day, so not a connection we count.