AA 5353 then AA 3927 at Charlotte
Wilmington → Charlotte → Charleston (South Carolina)
Timetable, Charlotte time
15:16AA 5353 from Wilmington at the gate
10:25AA 3927 to Charleston (South Carolina) leaves the gate
19 h 09 apart on the timetable, overnight
AA 3927 leaves 19 h 09 after AA 5353 arrives on the timetable: more than half a day, so not a connection we count.