AA 5353 then AA 5055 at Charlotte
Wilmington → Charlotte → Louisville
Timetable, Charlotte time
15:16AA 5353 from Wilmington at the gate
13:15AA 5055 to Louisville leaves the gate
21 h 59 apart on the timetable, overnight
AA 5055 leaves 21 h 59 after AA 5353 arrives on the timetable: more than half a day, so not a connection we count.