AA 5353 then AA 5388 at Charlotte
Wilmington → Charlotte → Washington National
Timetable, Charlotte time
15:16AA 5353 from Wilmington at the gate
11:25AA 5388 to Washington National leaves the gate
20 h 09 apart on the timetable, overnight
AA 5388 leaves 20 h 09 after AA 5353 arrives on the timetable: more than half a day, so not a connection we count.