AA 5353 then AA 5457 at Charlotte
Wilmington → Charlotte → Manchester
Timetable, Charlotte time
15:16AA 5353 from Wilmington at the gate
12:45AA 5457 to Manchester leaves the gate
21 h 29 apart on the timetable, overnight
AA 5457 leaves 21 h 29 after AA 5353 arrives on the timetable: more than half a day, so not a connection we count.