AA 5353 then AA 5490 at Charlotte
Wilmington → Charlotte → Dayton
Timetable, Charlotte time
15:16AA 5353 from Wilmington at the gate
10:35AA 5490 to Dayton leaves the gate
19 h 19 apart on the timetable, overnight
AA 5490 leaves 19 h 19 after AA 5353 arrives on the timetable: more than half a day, so not a connection we count.