AA 5353 then AA 5634 at Charlotte
Wilmington → Charlotte → Louisville
Timetable, Charlotte time
15:16AA 5353 from Wilmington at the gate
10:50AA 5634 to Louisville leaves the gate
19 h 34 apart on the timetable, overnight
AA 5634 leaves 19 h 34 after AA 5353 arrives on the timetable: more than half a day, so not a connection we count.