AA 5353 then AA 5820 at Charlotte
Wilmington → Charlotte → Roanoke
Timetable, Charlotte time
15:16AA 5353 from Wilmington at the gate
13:16AA 5820 to Roanoke leaves the gate
22 h apart on the timetable, overnight
AA 5820 leaves 22 h after AA 5353 arrives on the timetable: more than half a day, so not a connection we count.