AA 5353 then AA 5831 at Charlotte
Wilmington → Charlotte → Tri-Cities
Timetable, Charlotte time
15:16AA 5353 from Wilmington at the gate
13:10AA 5831 to Tri-Cities leaves the gate
21 h 54 apart on the timetable, overnight
AA 5831 leaves 21 h 54 after AA 5353 arrives on the timetable: more than half a day, so not a connection we count.