AA 5353 then AA 5843 at Charlotte
Wilmington → Charlotte → Roanoke
Timetable, Charlotte time
15:16AA 5353 from Wilmington at the gate
08:30AA 5843 to Roanoke leaves the gate
17 h 14 apart on the timetable, overnight
AA 5843 leaves 17 h 14 after AA 5353 arrives on the timetable: more than half a day, so not a connection we count.