AA 5353 then AA 5869 at Charlotte
Pensacola → Charlotte → Tri-Cities
Timetable, Charlotte time
08:15AA 5353 from Pensacola at the gate
20:39AA 5869 to Tri-Cities leaves the gate
12 h 24 apart on the timetable
AA 5869 leaves 12 h 24 after AA 5353 arrives on the timetable: more than half a day, so not a connection we count.