AA 5353 then AA 5904 at Charlotte
Wilmington → Charlotte → Knoxville
Timetable, Charlotte time
15:16AA 5353 from Wilmington at the gate
10:40AA 5904 to Knoxville leaves the gate
19 h 24 apart on the timetable, overnight
AA 5904 leaves 19 h 24 after AA 5353 arrives on the timetable: more than half a day, so not a connection we count.