AA 5353 then AA 591 at Charlotte
Wilmington → Charlotte → Denver
Timetable, Charlotte time
15:16AA 5353 from Wilmington at the gate
13:28AA 591 to Denver leaves the gate
22 h 12 apart on the timetable, overnight
AA 591 leaves 22 h 12 after AA 5353 arrives on the timetable: more than half a day, so not a connection we count.