AA 5353 then AA 5978 at Charlotte
Wilmington → Charlotte → Charlottesville
Timetable, Charlotte time
15:16AA 5353 from Wilmington at the gate
13:51AA 5978 to Charlottesville leaves the gate
22 h 35 apart on the timetable, overnight
AA 5978 leaves 22 h 35 after AA 5353 arrives on the timetable: more than half a day, so not a connection we count.