AA 5377 then AA 2276 at Charlotte
Rochester → Charlotte → Orlando
Timetable, Charlotte time
13:55AA 5377 from Rochester at the gate
10:47AA 2276 to Orlando leaves the gate
20 h 52 apart on the timetable, overnight
AA 2276 leaves 20 h 52 after AA 5377 arrives on the timetable: more than half a day, so not a connection we count.