AA 5377 then AA 2509 at Charlotte
Rochester → Charlotte → Chicago O'Hare
Timetable, Charlotte time
15:11AA 5377 from Rochester at the gate
14:54AA 2509 to Chicago O'Hare leaves the gate
23 h 43 apart on the timetable, overnight
AA 2509 leaves 23 h 43 after AA 5377 arrives on the timetable: more than half a day, so not a connection we count.