AA 5377 then AA 2725 at Charlotte
Rochester → Charlotte → Charleston (South Carolina)
Timetable, Charlotte time
15:11AA 5377 from Rochester at the gate
15:00AA 2725 to Charleston (South Carolina) leaves the gate
23 h 49 apart on the timetable, overnight
AA 2725 leaves 23 h 49 after AA 5377 arrives on the timetable: more than half a day, so not a connection we count.