AA 5377 then AA 5543 at Charlotte
Rochester → Charlotte → Pittsburgh
Timetable, Charlotte time
15:11AA 5377 from Rochester at the gate
08:35AA 5543 to Pittsburgh leaves the gate
17 h 24 apart on the timetable, overnight
AA 5543 leaves 17 h 24 after AA 5377 arrives on the timetable: more than half a day, so not a connection we count.