AA 5385 then AA 2255 at Charlotte
Lexington → Charlotte → Pittsburgh
Timetable, Charlotte time
10:48AA 5385 from Lexington at the gate
08:55AA 2255 to Pittsburgh leaves the gate
22 h 07 apart on the timetable, overnight
AA 2255 leaves 22 h 07 after AA 5385 arrives on the timetable: more than half a day, so not a connection we count.