AA 5390 then UA 3302 at Charlotte
Lexington → Charlotte → Rochester
Timetable, Charlotte time
09:22AA 5390 from Lexington at the gate
07:30UA 3302 to Rochester leaves the gate
22 h 08 apart on the timetable, overnight
UA 3302 leaves 22 h 08 after AA 5390 arrives on the timetable: more than half a day, so not a connection we count.