AA 5416 then AA 5488 at Charlotte
Atlanta (ATL) → Charlotte → Melbourne (Florida)
Timetable, Charlotte time
15:55AA 5416 from Atlanta (ATL) at the gate
15:21AA 5488 to Melbourne (Florida) leaves the gate
23 h 26 apart on the timetable, overnight
AA 5488 leaves 23 h 26 after AA 5416 arrives on the timetable: more than half a day, so not a connection we count.