AA 5416 then AA 6089 at Charlotte
Atlanta (ATL) → Charlotte → Wilmington
Timetable, Charlotte time
15:55AA 5416 from Atlanta (ATL) at the gate
14:35AA 6089 to Wilmington leaves the gate
22 h 40 apart on the timetable, overnight
AA 6089 leaves 22 h 40 after AA 5416 arrives on the timetable: more than half a day, so not a connection we count.