AA 5423 then UA 3441 at Charlotte
Greenville-Spartanburg → Charlotte → Washington
Timetable, Charlotte time
10:56AA 5423 from Greenville-Spartanburg at the gate
10:24UA 3441 to Washington leaves the gate
23 h 28 apart on the timetable, overnight
UA 3441 leaves 23 h 28 after AA 5423 arrives on the timetable: more than half a day, so not a connection we count.