AA 5426 then AA 2533 at Charlotte
Lafayette → Charlotte → Cincinnati
Timetable, Charlotte time
10:24AA 5426 from Lafayette at the gate
22:27AA 2533 to Cincinnati leaves the gate
12 h 03 apart on the timetable
AA 2533 leaves 12 h 03 after AA 5426 arrives on the timetable: more than half a day, so not a connection we count.