AA 5430 then AA 3309 at Charlotte
Dayton → Charlotte → Milwaukee
Timetable, Charlotte time
11:32AA 5430 from Dayton at the gate
10:40AA 3309 to Milwaukee leaves the gate
23 h 08 apart on the timetable, overnight
AA 3309 leaves 23 h 08 after AA 5430 arrives on the timetable: more than half a day, so not a connection we count.