AA 5440 then AA 5126 at Charlotte
Appleton → Charlotte → Louisville
Timetable, Charlotte time
10:34AA 5440 from Appleton at the gate
22:41AA 5126 to Louisville leaves the gate
12 h 07 apart on the timetable
AA 5126 leaves 12 h 07 after AA 5440 arrives on the timetable: more than half a day, so not a connection we count.