AA 5440 then AA 5220 at Charlotte
Appleton → Charlotte → Washington
Timetable, Charlotte time
11:35AA 5440 from Appleton at the gate
11:21AA 5220 to Washington leaves the gate
23 h 46 apart on the timetable, overnight
AA 5220 leaves 23 h 46 after AA 5440 arrives on the timetable: more than half a day, so not a connection we count.