AA 5440 then AA 5374 at Charlotte
Appleton → Charlotte → Pensacola
Timetable, Charlotte time
11:35AA 5440 from Appleton at the gate
11:10AA 5374 to Pensacola leaves the gate
23 h 35 apart on the timetable, overnight
AA 5374 leaves 23 h 35 after AA 5440 arrives on the timetable: more than half a day, so not a connection we count.