AA 5440 then AA 5486 at Charlotte
Appleton → Charlotte → Greenville-Spartanburg
Timetable, Charlotte time
11:35AA 5440 from Appleton at the gate
10:25AA 5486 to Greenville-Spartanburg leaves the gate
22 h 50 apart on the timetable, overnight
AA 5486 leaves 22 h 50 after AA 5440 arrives on the timetable: more than half a day, so not a connection we count.