AA 5453 then AA 1689 at Charlotte
Manchester → Charlotte → Sarasota
Timetable, Charlotte time
09:45AA 5453 from Manchester at the gate
08:20AA 1689 to Sarasota leaves the gate
22 h 35 apart on the timetable, overnight
AA 1689 leaves 22 h 35 after AA 5453 arrives on the timetable: more than half a day, so not a connection we count.