AA 5457 then AA 5601 at Charlotte
Manchester → Charlotte → Nashville
Timetable, Charlotte time
18:18AA 5457 from Manchester at the gate
17:57AA 5601 to Nashville leaves the gate
23 h 39 apart on the timetable, overnight
AA 5601 leaves 23 h 39 after AA 5457 arrives on the timetable: more than half a day, so not a connection we count.