AA 5459 then AA 2885 at Charlotte
Columbia Metropolitan → Charlotte → Jacksonville
Timetable, Charlotte time
17:25AA 5459 from Columbia Metropolitan at the gate
17:20AA 2885 to Jacksonville leaves the gate
23 h 55 apart on the timetable, overnight
AA 2885 leaves 23 h 55 after AA 5459 arrives on the timetable: more than half a day, so not a connection we count.