AA 5485 then AA 2559 at Charlotte
Daytona Beach → Charlotte → Detroit
Timetable, Charlotte time
20:56AA 5485 from Daytona Beach at the gate
20:51AA 2559 to Detroit leaves the gate
23 h 55 apart on the timetable, overnight
AA 2559 leaves 23 h 55 after AA 5485 arrives on the timetable: more than half a day, so not a connection we count.