AA 5485 then AA 2562 at Charlotte
Daytona Beach → Charlotte → St Louis
Timetable, Charlotte time
21:49AA 5485 from Daytona Beach at the gate
20:52AA 2562 to St Louis leaves the gate
23 h 03 apart on the timetable, overnight
AA 2562 leaves 23 h 03 after AA 5485 arrives on the timetable: more than half a day, so not a connection we count.