AA 5489 then AA 5069 at Charlotte
Daytona Beach → Charlotte → Cincinnati
Timetable, Charlotte time
14:19AA 5489 from Daytona Beach at the gate
13:41AA 5069 to Cincinnati leaves the gate
23 h 22 apart on the timetable, overnight
AA 5069 leaves 23 h 22 after AA 5489 arrives on the timetable: more than half a day, so not a connection we count.