AA 5490 then AA 2276 at Charlotte
Dayton → Charlotte → Orlando
Timetable, Charlotte time
14:32AA 5490 from Dayton at the gate
10:47AA 2276 to Orlando leaves the gate
20 h 15 apart on the timetable, overnight
AA 2276 leaves 20 h 15 after AA 5490 arrives on the timetable: more than half a day, so not a connection we count.