AA 5490 then AA 6052 at Charlotte
Dayton → Charlotte → Tallahassee
Timetable, Charlotte time
14:32AA 5490 from Dayton at the gate
14:20AA 6052 to Tallahassee leaves the gate
23 h 48 apart on the timetable, overnight
AA 6052 leaves 23 h 48 after AA 5490 arrives on the timetable: more than half a day, so not a connection we count.