AA 5540 then AA 5807 at Charlotte
Wilkes-Barre → Charlotte → Wilmington
Timetable, Charlotte time
09:29AA 5540 from Wilkes-Barre at the gate
08:35AA 5807 to Wilmington leaves the gate
23 h 06 apart on the timetable, overnight
AA 5807 leaves 23 h 06 after AA 5540 arrives on the timetable: more than half a day, so not a connection we count.