AA 5540 then AA 5843 at Charlotte
Wilkes-Barre → Charlotte → Roanoke
Timetable, Charlotte time
09:29AA 5540 from Wilkes-Barre at the gate
08:30AA 5843 to Roanoke leaves the gate
23 h 01 apart on the timetable, overnight
AA 5843 leaves 23 h 01 after AA 5540 arrives on the timetable: more than half a day, so not a connection we count.