AA 5543 then AA 2376 at Charlotte
Pittsburgh → Charlotte → Detroit
Timetable, Charlotte time
12:39AA 5543 from Pittsburgh at the gate
12:20AA 2376 to Detroit leaves the gate
23 h 41 apart on the timetable, overnight
AA 2376 leaves 23 h 41 after AA 5543 arrives on the timetable: more than half a day, so not a connection we count.