AA 5553 then AA 3201 at Charlotte
Knoxville → Charlotte → Charleston (South Carolina)
Timetable, Charlotte time
08:20AA 5553 from Knoxville at the gate
20:46AA 3201 to Charleston (South Carolina) leaves the gate
12 h 26 apart on the timetable
AA 3201 leaves 12 h 26 after AA 5553 arrives on the timetable: more than half a day, so not a connection we count.