AA 5553 then AA 3268 at Charlotte
Knoxville → Charlotte → Punta Cana
Timetable, Charlotte time
08:20AA 5553 from Knoxville at the gate
07:10AA 3268 to Punta Cana leaves the gate
22 h 50 apart on the timetable, overnight
AA 3268 leaves 22 h 50 after AA 5553 arrives on the timetable: more than half a day, so not a connection we count.