AA 5564 then AA 1176 at Charlotte
Harrisburg → Charlotte → Fort Myers
Timetable, Charlotte time
18:46AA 5564 from Harrisburg at the gate
15:39AA 1176 to Fort Myers leaves the gate
20 h 53 apart on the timetable, overnight
AA 1176 leaves 20 h 53 after AA 5564 arrives on the timetable: more than half a day, so not a connection we count.